(22d) Inverse Emulsion Synthesis of Hydrogel-Coated Gold Nanoshells for Protein Biomarker Quantification
Hydrogel nanoparticles were formed by an inverse emulsion copolymerization of methyacrylic acid with acrylamide or N-isopropylacrylamide, crosslinked with N,Nâ-methylenebis(acrylamide), and characterized by dynamic light scattering, potentiometric titration, and Fourier-transform infrared spectroscopy. AuNS were prepared using the method of seeded growth of gold colloids on aminated silica nanoparticles followed by encapsulation with poly(maleic anhydride-alt-1-octadecene)-g-poly(ethylene glycol) methacrylate (PMAO-g-PEGMA) graft copolymer to improve particle stability. Hydrogel-coated AuNS were prepared using the same nanoparticle synthesis method with the addition of PMAO-g-PEGMA AuNS to the aqueous phase of the inverse emulsion. UV-Vis spectroscopy was used to measure the shifts in the wavelength of the localized surface plasmon resonance for the hydrogel-coated AuNS. The successful synthesis of coated AuNS with both formulations shows immense promise for the creation of a library of hydrogels with various functional groups that could be used to alter affinity for different protein biomarkers from patient biofluids.
Work supported by National Institutes of Health Grant R01-EB022025