(217a) Purification of Monoclonal Antibodies Using a z2 Membrane Chromatography (z2MC) Module | AIChE

(217a) Purification of Monoclonal Antibodies Using a z2 Membrane Chromatography (z2MC) Module


Ghosh, R. - Presenter, McMaster University
Roshankhah, R., McMaster University
Pelton, R., McMaster University
Chen, G., McMaster University
Durocher, Y., NRC Montreal
Monoclonal antibodies (mAbs) comprise the largest category of biopharmaceuticals. Protein-A affinity chromatography is the standard method by which mAbs are purified. Protein-A resins are prohibitively expensive and the separation process involves mAb elution under acidic pH conditions, which could potentially destabilize the protein and promote its aggregation. Moreover, the protein-A ligand cannot distinguish between mAb charge variants. Also, aggregated forms of the mAb can bind to protein-A and therefore any aggregates present in the cell culture supernatant is co-eluted with the mAb. Hence, an alternative to protein-A based separation would be desirable. In this study, we examine mAb purification using two different types of chromatographic devices having equivalent volume: resin based (Capto S ImpAct) cation exchange chromatography column, and cation exchange z2 membrane chromatography or z2MC module, a novel separation device designed and developed within our group. The z2MC device addressed some of the problems associated with the resin-based column. For example, the product obtained with the z2MC device was purer than that obtained with resin based cation exchange chromatography and the separation process was significantly faster. In this presentation we highlight the advantages of using the z2MC module for high-resolution protein separations.