(165i) Toxicity of Magnetic Nanocomposites Using Zebrafish As a Biological Model
AIChE Annual Meeting
Thursday, November 19, 2020 - 8:00am to 9:00am
Toxicity was determined by means of hatching index, survival, malformation, and bioaccumulation of the nanocomposites in embryo of 8 hours post-fertilization and larvae of 4 days pos-fertilization. Additionally, behavior tests were performed to prove changes due to the presence of nanocomposites in the water. Magnetite nanoparticles were synthesized by the chemical co-precipitation method and different surface functionalizations were applied to obtained five different nanocomposites. (3-Aminopropyl) triethoxysilane (APTES), L-Cysteine (Cys) and 3-(triethoxysilyl) propylsuccinic anhydride (CAS) were used as functionalization molecules, thus, the five different nanocomposites corresponded magnetite nanoparticles (MNP), MNP+APTES, MNP+CAS, MNP+Cys, MNP+APTES+Cys, Egg Water was used as negative control and 3,4-Dichloroaniline as positive control. All these nanocomposites were design for the treatment of wastewater; however, in this work, the purpose was to evaluate the possible effects of toxicity on the animal model rather than their wastewater treatment effectiveness. All nanocomposites were evaluated at concentrations of 1, 10, 100 and 1,000 µg / ml, that included the protocol range of OECD (2013). Three replications were performed for each treatment with a (n) of 10 individual per nanocomposite and were exposed for 96 hours with treatments changes every 24 hours. Results showed that, at the concentration of 1,000 µg / ml the nanocomposites accumulated in the chorion delayed the hatching of the embryos by approximately 24 hours when compared to the other concentrations. At lower concentrations, although having accumulation of the nanocomposites on the chorion, hatching rate did not present a significant change compared the negative control. Both larvae and embryo tests showed a trend relating the increase of nanocomposite concentration with the decrease of the survival rate (up to 11 ± 1%) the increment in the number of malformations (up to 6 ± 1%) and the appearance of anxious behaviors (i.e. some groups showed a high sensitivity to light stimulation, while others did not react). Reproduction of the treated animals was also evaluated, and the results showed that none of the tested nanocomposites affected their reproduction. Therefore, it can be concluded that the tested nanocomposites could be a viable alternative in wastewater remediation. In general, the toxicity of these magnetic nanocomposites is low (between 3% and 12%), and considering that their final concentration, obtained after wastewater treatment and magnetic recovery, can be even lower than those tested, our results suggested their viable use in this type of applications. However, more detailed studies are recommended to determine internal bioaccumulation of nanocomposites in the animal model.
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