(160d) Degradable Catechin Particles As an Effective Antioxidant Material | AIChE

(160d) Degradable Catechin Particles As an Effective Antioxidant Material


Sahiner, N. - Presenter, Canakkale Onsekiz Mart Univ
Bhethanabotla, V., University of South Florida
Ayyala, R. S., University of South Florida
Mohapatra, S., University of South Florida
Suner, S., University of South Florida
Catechin (CAT) is a polyphenolic compound found in many fruits, vegetables and beverages such as strawberries, black grape, green tea and so on.1 The natural characteristics such as antioxidant, anti-inflammatory, anticancer, antimicrobial, antifibrotic properties make catechin an intriguing compound for numerous biomedical use.2-3 Therefore, CAT and its derivatives afford many therapeutic advantages in medical, food and cosmetic industries.1 Here, we present a single step synthesis of degradable CAT microparticles using trimethylolpropane triglycidyl ether (TMPGDE) as crosslinker at 100, 200, and 300% mole ratios based on CAT molecule in a microemulsion system. The hydrolytic degradation of CAT particles was investigated at physiological conditions e.g., at pHs 1.0, 7.4, and 9.0 at 37.5 °C. A sustainable and long-term CAT release kinetic was obtained for CAT particles prepared at 100% crosslinked TMPGDE with 264 mg/g CAT release within 10 days at pH 7.4 and 37.5 °C. The CAT particles possess potent antioxidant properties with 55.0±0.9 µg/mLgallic acid equivalency in terms of total phenol content and 0.88±0.3 µmol/g trolox equivalentantioxidant capacity. The blood compatibility of CAT particles was investigated via hemocompatibility tests with blood clotting and hemolysis assays and the cytotoxicity of CAT particles was determined on CCD841 normal colon cell line. It was found that CAT microparticles can be used for vascular system up to 10 µg/mLconcentration and induce no toxicity to normal colon cell line up to 250 µg/mLconcentration for 24 h incubation time. Therefore, the biodegradable CAT particles with natural origin has great potential in different biomedical fields owing to its’ sustainable and long-term CAT release capability, antioxidant behavior and lesser toxicity.


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