(157w) Biocompatibility and Potential Regenerative Effects of Sugarcane Derivatives in a 2D Skin Wound Model | AIChE

(157w) Biocompatibility and Potential Regenerative Effects of Sugarcane Derivatives in a 2D Skin Wound Model

Authors 

Muñoz Camargo, C. - Presenter, Universidad de los Andes
Cuellar, M., Universidad de los Andes
Serna, J. A., Universidad de los Andes
Rodriguez, J., Agrosavia
Escobar, S., Agrosavia
Cruz, J. C., Universidad de los Andes
Plant extracts have been widely used for medicinal purposes throughout the world for thousands of years. Many of their applications as therapeutic treatments have been derived from tradition and often have no fundamental scientific evidence or justification based on chemical composition [1]. In many rural areas of developing countries, traditional treatments continue to be the primary source of medicine due to 53-90% of people relying on this knowledge to meet their primary healthcare needs [2] [3] [4]. In particular, non-centrifugal sugarcane is traditionally used in Colombia as a healing and regenerative ointment for superficial skin wounds. The present study aims at providing scientific evidence for this long-lasting Colombian tradition by evaluating the wound healing potential of extracts isolated from sugarcane and its derivatives, which were fabricated under meticulously controlled thermal conditions as reported by Rodríguez et. al [5]. Sugarcane derivatives (juice, syrup, non-centrifugal sugarcane (NCS), sugarcane stalk) produced under the previously mentioned protocol and commercially available sugarcane produced under traditional methods were lyophilized to remove up to 95% of the water content. Potentially therapeutic extracts were isolated from all five samples using supercritical fluid extraction with methanol as co-solvent [6]. All samples (sugarcane-derivatives and extracts) were evaluated at a range of concentrations (1mg/mL to 31.25 µg/mL) for their cytotoxic and regenerative effects. Cell cytotoxicity was determined by MTT assays using Vero cells (CCL-081) [7], as well as, hemolysis [8] and platelet aggregation assays using donated human blood and plasma [9]. Sample concentrations with less than 30% cytotoxicity and less than 5% hemolytic activity were used to determine the effect on cell migration in a keratinocyte 2D scratch-wound assay. For this, the total area of the scratch wound was quantified by analyzing images taken in triplicate at 3 different time points (t = 0, 12 and 24 hours) using the open-access software ImageJ [10][11]. Results showed that sugarcane juice and syrup exhibit low cytotoxicity at concentrations below 125µg/mL and 62.5µg/mL, respectively. NCS showed no evidence of major cytotoxic effects under the concentrations evaluated. Methanol-based extracts isolated from all five samples can only be used under 10% (v/v) concentrations due to the well-known methanol’s cytotoxic effects in vitro. Moreover, none of the samples evaluated exhibited a hemolytic effect or significant platelet aggregation at the concentrations assayed. Figure 1 provides visual evidence of increased cell migration induced by the syrup sugarcane derivative when compared to the control (absence of treatment). In conclusion, the results presented in this study suggest that sugarcane and its derivatives exhibit great potential in the discovery of new therapeutic molecules for the development of novel and more effective wound healing treatments. Further studies must be performed; however, to properly identify specific therapeutic molecules, which may be responsible for the different effects hereby described.

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