Protein Engineering

New Technologies from Engineered Chimeric Proteins

SBE Webinar

Mar 21, 2011

David Wood

We engineer designer fusion proteins that combine domains and functions from unrelated proteins and enzymes.

Modular Biocatalysis

SBE Webinar

Jun 29, 2010

Chaitan Khosla

Modularity is a highly sought after feature in engineering design. A modular catalyst is a multi-component system whose parts can be predictably interchanged for functional flexibility and variety.

Breaking the Cell Wall Barrier for Difficult-to-Express Natural and Supernatural Products

SBE Webinar

May 27, 2010

James Swartz

Modern recombinant DNA expression technologies, by definition, challenge cells to engage in unnatural acts; specifically, the production of new types and large quantities of proteins for which the cells have not evolved.

Carbohydrate Circle and Genetic Information Flow Studies of Escherichia Coli Reveal the Molecular Mechanisms of Resistance to Balofloxacin

Nov 10, 2010

Xuan-Xian Peng

Protein-protein interactions are pivotal in many biological processes and essential for a global understanding of cell functions. In the present study, we combined 2-D native/SDS-PAGE-based proteomics with Co-immunoprecipitation, far-Western blotting, His-tag affinity purification and functional...

A Comprehensive Profiling of Genome-Wide Transcriptional Programs of Primary Hepatocytes in Vitro Cultures

Nov 8, 2010

Yeonhee Kim

The liver is one of most important organs in our bodies and performs many essential functions including metabolism, synthesis, secretion, detoxification, and storage. Hepatocytes are the principal cell type that accounts for 80% of liver and involved in major liver-specific functions. In vitro...

Modeling Eukaryotic Unfolded Protein Response and Its Role in Cellular Stress

Nov 7, 2010

Anirikh Chakrabarti

Malfunctions in the folding state of critical proteins have been linked with cancer, diabetes and other diseases. Proteins requiring complex post-translational modification are processed in the Endoplasmic Reticulum (ER). Cells monitor protein folding by an inbuilt quality-control system involving...

Purifying Therapeutic Monoclonal Antibodies

May

2008

SBE Special Section

Amit Mehta, Amy Len, Benedicte Lebreton, Bradley Wolk, Jace Fogle, Martha Lovato Tse, Nuno Fontes, Robert van Reis, Roshan Shrestha

Eliminating the affinity chromatography step in the mAb purification process trims equipment requirements, shrinks the plant footprint— and reduces costs.

Your Personal Genome for $1,000?

November

2009

SBE Special Section

IBM scientists from four fields — nanofabrication, microelectronics, physics and biology — are collaborating to develop a “DNA transistor” that could make obtaining a personalized genome analysis easier, faster, and cheaper — ultimately as low as $...

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Protein Engineering