New Technologies from Engineered Chimeric Proteins

SBE Webinar
Originally delivered Mar 21, 2011

We engineer designer fusion proteins that combine domains and functions from unrelated proteins and enzymes. In bioseparations, we have combined a previously developed pH-sensitive self-cleaving protein with a variety of purification tags to produce simple and economical methods for purifying recombinant proteins. In biosensing, we have developed allosteric proteins that cause Escherichia coli strains to become growth-dependent on endocrine active compounds. Remarkably, this genetically simple bacterial sensor can differentiate agonist from antagonist activities and has been effective in detecting estrogenicity in a wide variety of pollutants, home products and natural herbal remedies.

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