Browse Issue Articles in this Issue A novel counter - selection method for markerless genetic modification in Synechocystis sp. PCC 6803 Affinity chromatographic purification of human immunoglobulin a from chinese hamster ovary cell culture supernatant Avidity - mediated virus separation using a hyperthermophilic affinity ligand Biohydrogen production by dark fermentation of glycerol using Enterobacter and Citrobacter Sp Cell behavior observation and gene expression analysis of melanoma associated with stromal fibroblasts in a three - dimensional magnetic cell culture array Comparative analysis of heterologous expression, biochemical characterization optimal production of an alkaline α - amylase from alkaliphilic Alkalimonas amylolytica in Escherichia coli and Pichia pastoris Cryopreservation of pluripotent stem cell aggregates in defined protein - free formulation Development of large engineered cartilage constructs from a small population of cells Direct and indirect use of GFP whole cell biosensors for the assessment of bioprocess performances: Design of milliliter scale - down bioreactors Downstream processing, characterization, and structure - function relationship of solvent - , detergent - , psychro - , thermo - , alkalistable metalloprotease from metal - , solvent - tolerant psychrotrophic Pseudomonas putida SKG - 1 isolate Enhanced protein stability through minimally invasive, direct, covalent, and site - specific immobilization Enzymatic processes for the purification of trehalose Fed - batch CHO cell t - PA production and feed glutamine replacement to reduce ammonia production Hydrodynamic characteristics and microalgae cultivation in a novel flat - plate photobioreactor Immobilization of Oenococcus oeni in lentikats® to develop malolactic fermentation in wines Impact of aeration strategy on CHO cell performance during antibody production Improving the fermentation performance of saccharomyces cerevisiae by laccase during ethanol production from steam - exploded wheat straw at high - substrate loadings In vitro and in vivo evaluation of L - lactide/ε - caprolactone copolymer scaffold to support myoblast growth and differentiation Matrix effects during monitoring of antibody and host cell proteins using attenuated total reflection spectroscopy Model - based analysis on the extractability of information from data in dynamic fed - batch experiments Optimization of a two - step process comprising lipase catalysis and thermal cyclization improves the efficiency of synthesis of six - membered cyclic carbonate from trimethylolpropane and dimethylcarbonate Optimizing capacity utilization by large scale 3000 L perfusion in seed train bioreactors Osteogenic differentiation and osteochondral tissue engineering using human adipose - derived stem cells Phosphatidylcholine enrichment with medium chain fatty acids by immobilized phospholipase A1 - catalyzed acidolysis Process model comparison and transferability across bioreactor scales and modes of operation for a mammalian cell bioprocess Production and scale - up of a monoclonal antibody against 17 - hydroxyprogesterone Progenitor cells from cartilage-No osteoarthritis - grade - specific differences in stem cell marker expression Real - time monitoring of influenza virus production kinetics in HEK293 cell cultures Recombinant expression and purification of the antimicrobial peptide magainin - 2 Redox - enzymes, cells and micro - organisms acting on carbon nanostructures transformation: A mini - review Shrimp (Litopenaeus vannamei) trypsinogen production in Pichia pastoris bioreactor cultures Solubility properties and diffusional extraction behavior of natamycin from streptomyces gilvosporeus biomass The effect of ultrasonication on the immunomodulatory activity of low - quality ginseng