Hydrolysis kinetics were analyzed using a Yellow Springs Instruments (YSI Corp.) glucose analyzer. Enzyme kinetics were measured at different dilutions of biomass hydrolysate, with all three sources of biomass causing inhibition of the enzymes to different degrees. Alkaline pretreatments were observed to cause less enzyme inhibition than acidic pretreatments. It was noted that the presence of soluble oligomers in the hydrolysate resulted in apparent diminution of the inhibitory effect, resulting from an increased release of oligomer-derived glucose at higher concentrations of hydrolysate. Correction for this glucose contribution from the oligomeric sugars rendered the inhibitory effect of the hydrolysate more apparent.
Analytical interrogation of the hydrolysates quantified 40 different compounds present in the hydrolysates. Comparison of analytical to hydrolysis results indicate that enzyme inhibition can be correlated to the presence of select degradation compounds present in the hydrolysate.
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