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In this webinar, Prof. Su’etsugu will talk about a novel tool kit for life science researchers, “OriCiro™ Cell-free Cloning System.” Many life science laboratories perform a conventional DNA cloning relying on E. coli which has been developed almost fifty years ago. The E. coli cloning is time-consuming, labor-intensive and has a limitation in efficiency and applicable size. Some DNA sequences are difficult to get a clone due to a cell-toxicity. How can you streamline your cloning workflow and widen the scope of your research?
The OriCiro™ Cell-free Cloning System consists of two enzymatic reactions, Assembly and Amplification. The Assembly reaction allows seamless assembly of multiple overlapping DNA fragments (>50 fragments) in a one-step reaction. The assembly products including assembly intermediates can be added directly to the Amplification reaction to amplify selectively your target circular DNA like a plasmid. After the reaction (within several hours), you’ll get a high concentration of the circular DNA (up to 100 ng/µL) with a great purity. Because a whole replication cycle of the E. coli chromosome is reconstituted in vitro using 26 purified proteins, the Amplification reaction has a unique feature in amplification fidelity (a single error/100-Mb/cycle) and amplification size (up to 50-kb in the commercial version). Any sequences including GC-rich or cell-toxic sequences are applicable.
You’ll learn the OriCiro™ Cell-free Cloning System as a powerful, rapid and easy tool-kit for your DNA construction/amplification in the era of synthetic biology.
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