LIMITED TIME OFFER: Claim a 20% discount on eLearning courses with code ELEARN20. Offer is valid from April 1-30. Credential programs excluded from promo.
Discovery of new antibodies for therapeutics requires discerning tools to identify rare cells producing specific antibodies among many similar ones. Bioprocess development also relies on methods to identify optimized cell lines producing heterologous proteins. This talk will describe an approach for integrated single-cell analysis that allows high-throughput screening (10^4–10^5 cells per screen) for novel antibodies and productive clones. The approach uses a modular collection of techniques employing microfabricated arrays of subnanoliter containers to enable flexible, reconfigurable processes that facilitate the discovery of novel antibodies and evaluation of clonal lines used in manufacturing heterologous proteins. The application of this nanowell-based technology to screening hybridomas and other antibody-secreting cells will be presented. The use of the same platform to identify high-producing strains for heterologous proteins will also be presented. How these measures on clonal lines can also provide insight into the biological factors that impede the recovery of stable production lines in such processes will also be discussed.
Webinar content is available with the kind permission of the author(s) solely for the purpose of furthering AIChE’s mission to educate, inform and improve the practice of professional chemical engineering. All other uses are forbidden without the express consent of the author(s). For permission to re-use, please contact chemepermissions@aiche.org.
AIChE Practice+ provides learners with opportunities to work on real-world challenges through industry internships and competitions.
With AIChE Career Discovery®, we'll help you to identify aptitudes and skills you’ll need in order to achieve your full potential at various career stages.
AIChE Credential validates your proficiency with potential employers in areas such as process intensification, safety, sustainability and others.