Thermo Fisher Scientific, Inc.

HPLC Method Development Calculator
Many chromatographers are considering moving their current high-performance liquid chromatography (HPLC) methods to new column geometries with different particle sizes to improve speed, resolution, and/or sensitivity. The HPLC Method Development Calculator reduces the time needed to experimentally determine the most favorable analysis conditions by providing users with quick and simple access to the knowledge of the company’s chromatography experts. The interactive collection of online tools allows the user to calculate initial conditions for both gradient and isocratic methods. A liquid chromatography column selection tool aids in choosing an appropriate column for any application. The online tools are part of the Thermo Scientific Chromatography Resource Center

Nunc roller bottles
Nunc roller bottles for adherent cell culture applications are now available in glycol-modified polyethylene terphthalate (PETG) and polystyrene (PS). InVitro PETG roller bottles withstand freezing and thawing and come with either a smooth or pleated internal surface. TufRol PS roller bottles feature vertical pleats for easy emptying, and are indented to allow single-handed manipulation. The TufRol bottles can be used with polystyrene Nunc T-flasks and cell factory systems to create an integrated bioprocess. Nunc roller bottles can be further specified to include vented closures to facilitate gas exchange or poly-d-lysine for temperamental cells. All the bottles are manufactured in facilities that comply with current Good Manufacturing Practices (cGMP), ISO 9001:2008, and ISO 13485:2003 quality standards, of polymers that are certified USP Class VI. They are packaged and shipped under sterile conditions and printed with a lot number to ensure traceability.

TraceGold GC column
The TraceGold GC column comes with an integrated SafeGuard column, and is suitable for carrying out analytical investigations in environmental, food, petrochemical, forensic, and other applications. The system combines an analytical gas chromatography (GC) column with a built-in guard column, which is designed to extend the life of the analytical column and ensure consistency of results over a longer period of time, without compromising its chromatographic performance. The stainless steel system is available in a range of guard column lengths and analytical column specifications, and handles temperatures ranging from –60°C to 350°C.

Thermo Scientific iTEVA Precision software module
The Thermo Scientific iTEVA Precision software module enables high-precision, automated analysis of high-concentration elements and precious metals. It seamlessly integrates with the iCAP 6000 Series of inductively coupled plasma optical emission spectrometers (ICP-OES). The module features advanced data processing, which routinely enables relative standard deviation (RSD) values of less than 0.3% between sample aliquots to be achieved. In addition, the powerful automated data processing capability makes labor-intensive external calculations unnecessary and eliminates the potential for human transcription errors associated with performing manual offline calculations. Another benefit is the software’s ability to perform required post-run data reprocessing without re-analyzing samples, which accelerates workflows and conserves high-value samples. The software provides flexible options for unit sets that are user-definable on a per-analyte basis. Customizable data-filtering and reporting tools are also available, enabling users to maintain their preferred workflow while automating current sample-analysis processes. In addition, the software offers multi-language support, including English, German, French, Chinese, Japanese, and Russian.

Syncronis HPLC column
When developing a new method, one of the chromatographer’s key goals is to achieve a consistent, reproducible separation, and selecting a highly reproducible high-performance liquid chromatography (HPLC) column is essential to attaining this goal. The Syncronis HPLC column has been engineered to deliver consistent, predictable separations, from one run to the next and from one column to another. It features high-purity, high-surface-area silica, dense bonding, and double endcapping. Enhanced, automated column-packing methods used in manufacturing the Syncronis product line ensure consistency, and every column is individually tested to verify that it meets the required specifications. Syncronis columns are available with 5-µm particles for conventional HPLC applications, and with 1.7-µm particles for high-speed, high-efficiency ultra-HPLC separations.

Spectronic 200
The Spectronic 200, a new visible spectrophotometer, incorporates the latest optical and detector technologies to meet the needs of academic and industrial quality-control laboratories. This cost-effective instrument is simple to set up and use, requires little maintenance, has no moving parts, and has a small footprint. It delivers fast, full-wavelength scan data and multi-standard quantitative analysis. For users of the company’s predecessor Spectronic 20 and Genesys 20 spectro-photometers, the onboard software platform and simple user interface make upgrading easy.

Hypersil Gold hydrophilic interaction liquid chromatography columns
This company’s new line of Hypersil Gold hydrophilic interaction liquid chromatography (HILIC) columns are used for the separation of highly polar compounds, which are notoriously difficult to analyze using standard reversed-phase chromatography. The columns work by incorporating water in the highly organic mobile phase to form an adsorbed water-rich layer on the polar stationary phase surface into which analyte molecules partition. Retention is governed by dipole-dipole interactions and hydrogen bonding mechanisms. The highly organic mobile phase contains low salt levels, making Hypersil Gold HILIC columns ideal for use with electrospray mass spectroscopy. With the ability to retain highly polar and hydrophilic compounds, this new HILIC range of columns has been developed to aid the analysis of compounds that are traditionally difficult to retain using conventional C18 columns. The columns are available with 1.9-, 3- and 5-micrometer particle sizes.

GRAMS spectroscopy software
GRAMS spectroscopy software helps in the visualizing, processing, reporting and managing of spectroscopy data. Version 9.0 is comprised of complementary and fully integrated applications and modules centered on the core GRAMS/AI spectroscopy data processing and reporting software. It has advanced processing routines, as well as data comparison and visualization features. It can handle data from any analytical instrument. Its intuitive and simplified workflows enable scientists to more easily and rapidly access data and make more informed decisions about the results generated. GRAMS 9.0 introduces GRAMS IQ, which enhances the software’s visual chemometric modeling abilities. This simplifies visual workflow and model deployment functions for quantitative and qualitative analyses. The Smart Convert/Import feature has an interactive import wizard that enables scientists to manage data from more than 180 types of infrared spectroscopy (IR), ultraviolet and visible spectroscopy (UV-Vis), and liquid/gas chromatography with mass spectrometry (LC/GC-MS) instruments.

GENESYS 10S Vis, the GENESYS 10S UV-Vis, and the BioMate 3S spectrophotometers
The GENESYS 10S Vis, the GENESYS 10S UV-Vis, and the BioMate 3S spectrophotometers provide instrument control using embedded software that is strong enough for demanding analysis, but simple and straightforward enough for handling routine samples. They feature a front-panel USB port for method and data storage on removable memory devices, as well as printer and computer connectivity. Their easy-to-read liquid crystal display (LCD) is backlit for lower power usage and longer product life. The user-friendly software is driven from an integrated, chemical-resistant keypad. These spectrophotometers acquire scan data at up to 4,200 nm/min and are suitable for use in a wide variety of laboratory experiments, including wavelength scanning, qualitative analysis, kinetics, and fixed-wavelength measurements. The BioMate 3S uses the same hardware as the others, and offers additional flexibility with built-in biological test methods.

Masterflex P/S line of digital peristaltic pumping systems
The Masterflex P/S line of digital peristaltic pumping systems provides repeatability of 0.1% to maximize productivity in precision chemical feed, batch dispensing, and filling applications. The line covers a wide range of complete systems, which include a standard digital drive, a computerized drive, and a stainless steel National Electrical Manufacturers Association (NEMA) 4X process drive. Turndown ratios of up to 6,000:1 provide users with flexibility in both volume and time of dispensing. The pumps handle flow capacities from 0 to 54 gal/h, and can achieve a flow range of 380,000:1 in one pump head. The pumps are self-priming and able to operate dry. They are suitable for use with most chemicals, and contain no valves or seals. Fluid contacts only the tubing, providing contamination-free pumping. 

Lab Forms 2.5
Lab Forms 2.5 is a suite of gas chromatography / mass spectrometry (GC/MS) software designed for applications in environmental, clinical research and forensic toxicology, food safety, and general QA/QC laboratories. The suite includes three analysis and reporting software components. QuanLab Forms enables users to perform high-throughput quantitative and semi-quantitative GC/MS experiments. EnviroLab Forms assists environmental laboratories in balancing high productivity and throughput with strict quality-control standards. ToxLab Forms provides an intuitive, workflow-oriented approach to GC/MS acquisition, analysis and reporting in clinical research or forensic toxicology.

ChromQuest 5.0
ChromQuest 5.0 updates the previous Chromatography Data System (CDS) program with a new user interface, and data acquisition, processing and reporting methods that are designed to work with sampling and measurement instrumentation. This software can perform multi-level calibrations, graphic method editing, conditional sequence actions and automated LIMS (laboratory information management systems) export. It provides digital control for system components, such as pumps and detectors. Integrated instrument control supports the Focus GC, the Trace GC Ultra, and the TriPlus autosampler for liquid, headspace and solid-phase micro extraction (SPME) sampling techniques.

Nicolet iS 10 FT-IR spectrometer
The Nicolet iS 10 FT-IR spectrometer carries out an efficient analysis of both raw materials and finished goods. Integrated with OMNIC Specta software, the system allows users to identify individual components in a mixture from a single spectrum. Materials are identified rapidly and with complete confidence, which is backed by the device’s Spectral Performance Verification (SPV) feature, which regularly monitors the system’s accuracy and performance. This diagnostic can be performed overnight so that the system is ready for the next day. The spectrometer can be configured with the Nicolet iZ 10 module, which allows the simultaneous installation of two accessories, such as an integrating sphere or transmission compartment.

Nautilus LIMS version 8.0
Nautilus LIMS version 8.0 now includes data management functionality. Capable of capturing, archiving and storing both derived and raw instrument data, the data manager is a complete storage and retrieval system that understands and preserves analytical data, documents and PDF files. It provides instant access to data, enabling users to view, search and manipulate real data and metadata without requiring the original Nautilus software itself. The module converts instrument data to a vendor-neutral XML format, and allows linking to LIMS entities for instant investigation of suspect results.

Total Sulfur (TS) 4000 analyzer
The Total Sulfur (TS) 4000 analyzer incorporates true total sulfur (TTS) technology, which removes nitrogen interference and provides true total sulfur results, down to trace levels. Detectors typically identify nitrogen as nitrogen oxide because it emits at the same wavelength as sulfur dioxide, resulting in inaccurate analysis. This analyzer is suitable for a range of refinery and petroleum sample types with high nitrogen concentrations. TTS technology incorporates the same ASTM D5453 methodology used by the Environmental Protection Agency to formulate regulations limiting the sulfur content in automotive fuels. All TS 4000 analyzers feature the NeXYZ liquid universal autosampler, which offers quick automatic sample introduction and can be used for solids as well as direct and indirect injection of small volumes of hydrocarbons.

“Choosing the Right Instrument Vendor for Complete, Cost-Effective Compliance”
Titled “Choosing the Right Instrument Vendor for Complete, Cost-Effective Compliance,” this 19-page publication provides a thorough understanding of compliance basics to help laboratory managers, chief scientists and regulatory specialists determine the real value of an instrument vendor’s compliance capabilities and better evaluate their spectroscopy systems. Additionally, it details available processes for instrument validation, qualification and verification, while also mapping out these processes in highly regulated (cGMP, FDA), moderately regulated (GLP, ISO) and self-regulated (Six Sigma) manufacturing environments. It is available free-of-charge via www.thermo.com/comply.